Complimentary Silencers

There is a serious lack in affordable drug that can be used  both against the Covid-19 as a specific therapeutic and as a rapid diagnostic tool.

We are proposing a drug which is mixture of methylated antisense RNA strands with green fluorescent  protein tag along with the enzyme Human DICER.

This product can be easily made in any biochemistry laboratory and will be an all-in-one solution for Covid-19 pandemic.

There is a serious lack in affordable drug that can be used  both against the Covid-19 as a specific therapeutic and as a rapid diagnostic tool.

Covid-19, commonly known as Coronavirus, is a single-stranded positive-sense RNA virus. It is a known fact that RNA-duplex is thermodynamically more stable than ds-DNA which in turn is more stable than ss-RNA i.e. it requires more harsh conditions (Like higher temperature) to denature ds-RNA than ds-DNA. So, injecting a modified anti-sense RNA would effectively arrest RNA proliferation by forming a near-neutral duplex (i.e. this Duplex can't be proofread stopping the retrosynthesis) in a Corona-affected patient.

First of all, to synthesize the bulk amount of antisense RNA of covid-19, Reverse Transcriptase Polymerase chain reaction (RT-PCR) to be employed. It is methylated to hinder proof-reading and synthesizing some unwanted peptide. A Green-fluorescent protein(GFP) tag is attached to it.

Then, this antisense RNA to be introduced to empty caspids of retrovirus. These modified RNA virus would be then injected to patient along with human DICER enzyme, preferably to infected region of patients, like lungs.

These viral vectors will introduce these modified RNA to cells. in presence of COVID-19 RNA, it would bind to silence it. The duplex formed would be digested by DICER enzyme.

In absence of virus, the GFP tag would be translated. The fluorescenceanalysis of blood sample give viral-load of patient.  

Our drug is all-in-one solution so it would greatly reduce the total time and cost of each services like diagnosis and treatment.

Being made of available items, it is very feasible to make and is of superior specificity than any other drug or testing kit available in the current market

Our drug is all-in-one solution so it would greatly reduce the total time and cost of each services like diagnosis and treatment.

Being made of available items, it is very feasible to make and is of superior specificity than any other drug or testing kit available in the current market

Our non-invasive diagnostic tool will be able to detect COVID-19 infection in record time and and possibly the lowest cost available in the market. The low cost is because the dye and enzymes involved are regular laboratory reagents(such as DNAse I and ethidium bromide). The anti-sense RNA will also ensure that there are no false positives(as it is specific to the positive-sense SARS-CoV 2 RNA). The production of the anti-sense RNAs is done while preparing the diagnostic solution so would not delay the results. It is also cheap. The human dicer enzyme to be used as part of the therapeutic drug is also readily available.

Silencing gene expression using antisense RNA and si-RNA technology.

Detecting ds-RNA formed by the anti-sense RNA using fluorescent ds-RNA intercalating dyes. 

Antisense RNA technology is already implemented in current research and yet to developed as reported in “Preparation of dsRNA for microinjection experiments in mouse. - Cold Spring Harb Protoc. 2009 Jan;2009(1): pdb.prot5131”

The solution being at a concept stage, members are still being recruited whoever willing to contribute to the project.

The RNA based drug is tested computationally using:

1)  Random-walk model by Riddhi Manna

2)  Genetic Model by Pritam Sil

The circuit blueprint and autocad model of the automated drug-administering device is developed by Shubham Roy.

Our project consists of a rapid diagnostic tool and a therapeutic drug against COVID-19. It would be a self-sustaining as a business model as the revenue generation for the next batch of medicine or kits(both are part of our project) would be derived from the earlier batch's sales. A part of the revenue may be used for research and development or providing free kits or medicine to the economically challenged. We would like to collaborate with some organization who are already mass producing medicines or diagnostic kits.

Our project would be a collaboration with an organization that does mass production of diagnostic kits or medicines. The revenue generated would be able to continue the production of the existing diagnostic tool or medicine while a part of the revenue can be used to further research on the project.

We believe our project is an all-round potential solution for COVID-19. Before we can market our idea as a product in market,we require in-vivo testing our RNA-based drug, which couldn't be tested for being in a lockdown state.

SOLVE is the best platform to pitch our idea to leading professionals of the medical field and would help us connect with them. 

The prize money would definitely help to implement our idea immediately.

We are looking forward to partnering with some biotechnogical company or pharmaceutical company where we can effectively implement our project.